An automated p-nitrophenylphosphate serum alkaline phosphatase procedure for the "Robot Chemist".

A new, precise, and accurate procedure is described for the automated determination of serum alkaline phosphatase on the Robot Chemist. The substrate, p-nitrophenylphosphate in 2-amino-2-methyl-1-propanol, offers the prime advantage of providing directly its own chromogen, p-nitrophenol, following enzyme action. Other advantages are the small sample volumes required and linear reaction rates. Correlations are presented between the manual procedure and those used with both the Technicon AutoAnalyzer and the Robot Chemist.